首页  |  细胞库简介  |  细胞系目录  |  试剂类产品  |  细胞质量控制  |  订购和付款方式  |  管理团队  |  细胞培养基本方法  |  联系我们

中国科学院细胞库细胞株信息

点击订购此细胞株

细胞名称
Cell Name

HeLa人宫颈癌细胞

描述
Description

角蛋白免疫过氧化物酶染色阳性。 有报告称MS751细胞含有人乳头状瘤病毒18 (HPV-18)序列。 据报道,p53表达水平低,pRB(成视网膜细胞瘤抑制因子)表达水平正常。 在本库通过支原体检测。 在本库通过STR检测。

动物种别
Organism

性别
Gender

组织来源
Tissue and Cell Type

宫颈腺癌

形态
Morphology

上皮细胞

培养基和添加剂
Complete Growth Medium and Culture Conditions

MEM培养基(GIBCO,货号41500034,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。

供应限制
Permits and Restrictions
A。仅供研究之用。

REFERENCE

American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed. Washington, DC: American Public Health Association; 1992. AOAC International. Invasiveness by Escherichia coli of mammalian cells, microbiological method. Gaithersburg, MD: AOAC International; AOAC Official Method 982.36. U.S. Food & Drug Administration. Tests for enteroinvasive E. coli (EIEC). In: , editors. Bacteriological analytical manual. 8th ed. Gaithersburg, MD: AOAC International; 1995, p. 4.16. Baldi A , et al. Genomic structure of the human retinoblastoma-related Rb2/p130 gene. Proc. Natl. Acad. Sci. USA 93: 4629-4632, 1996. PubMed: 8643454 Gey GO , et al. Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium. Cancer Res. 12: 264-265, 1952. Chen TR . Re-evaluation of HeLa, HeLa S3, and Hep-2 karyotypes. Cytogenet. Cell Genet. 48: 19-24, 1988. PubMed: 3180844 Boshart M , et al. A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO J. 3: 1151-1157, 1984. PubMed: 6329740 Schneider-Gadicke A , Schwarz E . Different human cervical carcinoma cell lines show similar transcription patterns of human papillomavirus type 18 early genes. EMBO J. 5: 2285-2292, 1986. PubMed: 3023067 Schwarz E , et al. Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature 314: 111-114, 1985. PubMed: 2983228 Pater MM , Pater A . Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153 Yee C , et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 Scheffner M , et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218 Jones HW Jr, et al. George Otto Gey. (1899-1970). The HeLa cell and a reappraisal of its origin. Obstet. Gynecol. 38: 945-949, 1971. PubMed: 4942173 Proc. Soc. Exp. Biol. Med. 87: 480, 1954. J. Exp. Med. 97: 695, 1953. Fang X , et al. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. J. Biol. Chem. 272: 13683-13689, 1997. PubMed: 9153219 Bruder JT , Kovesdi I . Adenovirus infection stimulates the Raf/MAPK signaling pathway and induces interleukin-8 expression. J. Virol. 71: 398-404, 1997. PubMed: 8985363 Huber M , et al. Tyrosine phosphorylation events during coxsackievirus B3 replication. J. Virol. 71: 595-600, 1997. PubMed: 8985388 Olson JK , et al. Varicella-zoster virus Fc receptor gE glycoprotein: serine/threonine and tyrosine phosphorylation of monomeric and dimeric forms. J. Virol. 71: 110-119, 1997. PubMed: 8985329 Goodrum FD , Ornelles DA . The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383 Loffler S , et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 Hendricks DT , et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 Hoppe HC , et al. Identification of phosphatidylinositol mannoside as a mycobacterial adhesin mediating both direct and opsonic binding to nonphagocytic mammalian cells. Infect. Immun. 65: 3896-3905, 1997. PubMed: 9284169 Rieder G , et al. Role of adherence in Interleukin-8 induction in Helicobacter pylori-associated gastritis. Infect. Immun. 65: 3622-3630, 1997. PubMed: 9284128 St. Geme JW , et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Mansky LM . The mutation rate of human immunodeficiency virus type 1 is influenced by the vpr gene. Virology 222: 391-400, 1996. PubMed: 8806523 Dobbelstein M , Shenk T . Protection against apoptosis by the vaccinia virus SPI-2 (B13R) gene product. J. Virol. 70: 6479-6485, 1996. PubMed: 8709286 Churchill MJ , et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194 Goodrum FD , et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Kolanus W , et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Gan W , Rhoads RE . Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663 Koumenis C , Giaccia A . Tranformed cells require continuous activity of RNA polymerase II to resist oncogene-induced apoptosis. Mol. Cell. Biol. 17: 7306-7316, 1997. PubMed: 9372962 You M , et al. ch-IAp1, a member of the inhibitor-of-apoptosis protein family, is a mediator of the antiapoptotic activity of the v-Rel oncoprotein. Mol. Cell. Biol. 17: 7328-7341, 1997. PubMed: 9372964 Hess MT , et al. Base pair conformation-dependent excision of benzo{a}pyrene diol epoxide-guanine adducts by human nucleotide excision repair enzymes. Mol. Cell. Biol. 17: 7069-7076, 1997. PubMed: 9372938 Bartz SR , et al. Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G2 accumulation by a mechanism which differs from DNA damage checkpoint control. J. Virol. 70: 2324-2331, 1996. PubMed: 8642659 Roller RJ , et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 8627758 Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Anderson SM , et al. Intercellular transfer of a glycoslphosphatidylinositol (GPI)-linked protein: release and uptake of CD4-GPI from recombinant adeno-associated virus-transducted HeLa cells. Proc. Natl. Acad. Sci. USA 93: 5894-5898, 1996. PubMed: 8650189 Dittrich E , et al. A di-leucine motif and an upstream serine in the interleukin-6 (IL-6) signal transducer gp130 mediate ligand-induced endocytosis and down-regulation of the IL-6 receptor. J. Biol. Chem. 271: 5487-5494, 1996. PubMed: 8621406 Lee JH , et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Duus KM , Grose C . Multiple regulatory effects of varicella-zoster virus (VZV) gL on trafficking patterns and fusogenic properties of VZV gH. J. Virol. 70: 8961-8971, 1996. PubMed: 8971025 Lieber A , et al. Recombinant adenoviruses with large deletions geneerated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J. Virol. 70: 8944-8960, 1996. PubMed: 8971024 Yang C , Compans RW . Analysis of the cell fusion activities of chimeric simian immunodeficiency virus-murine leukemia virus envelope proteins: inhibitory effects of the R peptide. J. Virol. 70: 248-254, 1996. PubMed: 8523533 Schubert U , et al. The two biological activities of human immunodeficiency virus type 1 Vpu protein involve two separable structural domains. J. Virol. 70: 809-819, 1996. PubMed: 8551619 McKnight KL , Lemon SM . Capsid coding sequence is required for efficient replication of human rhinovirus 14 RNA. J. Virol. 70: 1941-1952, 1996. PubMed: 8627720 Lu FM , Lux SE . Constitutively active human notch 1 binds to the transcription factor CBF1 and stimulates transcription through a promoter containing a CBF1-responsive element. Proc. Natl. Acad. Sci. USA 93: 5663-5667, 1996. PubMed: 8643633 Yamaguchi Y , et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 Hewitt SM , et al. Differential function of Wilms' tumor gene WT1 splice isoforms in transcriptional regulation. J. Biol. Chem. 271: 8588-8592, 1996. PubMed: 8621487 Kotanides H , Reich NC . Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene. J. Biol. Chem. 271: 25555-25561, 1996. PubMed: 8810328 Zhao Z , et al. Activation of mitogen-activated protein (MAP) kinase pathway by pervanadate, a potent inhibitor of tyrosine phosphatases. J. Biol. Chem. 271: 22251-22255, 1996. PubMed: 8703041 Quitschke WW , et al. The initator element and proximal upstream sequences affect transcriptional activity and start site selection in the amyloid beta-protein precursor promoter. J. Biol. Chem. 271: 22231-22239, 1996. PubMed: 8703038 Hocking AM , et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651 33167: Chen H , et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022 Macville M , et al. Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping. Cancer Res. 59: 141-150, 1999. PubMed: 9892199 MOLECULAR THERAPY 11(4):531,2005

中文名称
人宫颈癌细胞
价格
Transmittal Fee
1400



您是第 13922559 位访问者,欢迎!
中国科学院典型培养物保藏委员会细胞库/中国科学院上海生命科学研究院细胞资源中心 版权所有
沪ICP备05033115号