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中国科学院细胞库细胞株信息

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细胞名称
Cell Name

Caco-2人结直肠腺癌细胞

描述
Description

这株细胞分离自直肠原位癌。 当长到满时,细胞表现出特征性的肠上皮细胞分化。 Caco-2细胞表达维甲酸结合蛋白I和维甲酸结合蛋白II,并呈角质蛋白阳性。 在本库通过支原体检测。 在本库通过STR检测。

动物种别
Organism

性别
Gender

组织来源
Tissue and Cell Type

结肠,结直肠腺癌

形态
Morphology

上皮细胞

培养基和添加剂
Complete Growth Medium and Culture Conditions

MEM培养基(GIBCO,货号41500034,添加NaHCO3 1.5g/L,Sodium Pyruvate 0.11g/L),80%;优质胎牛血清,20%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。

供应限制
Permits and Restrictions
A。仅供研究之用。

REFERENCE

Didier, E.S., et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDSpatients. J. Eukaryot. Microbiol 43: 34-43. 1996 PubMed:96151459 Jumade, C., and Malo, C. Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J. Cell. Physiol. 149: 24-33, 1991 PubMed: 92042315 Fogh, J., et al. Absence of HeLa ceil contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977 PubMed: 77097006 Fogh, J., et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977 PubMed:77210034 Adachi, A., et al. Productive, persistent infection of human co]orectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987 PubMed: 87061242 Trainer, D.L., et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296. 1988 PubMed:88114170 Cohen, M.B., et al. Receptors for Escherichia coil heat stable enterotoxin in human intestine and in a human intestinal cell line (Caco-2). J. Cell, Physiol. 156: 138-144,1993 PubMed: 93390921 Basson, M.D., et al. Effect of tyrosine kinase inhibition on basal and epidermal growth factor-stimulated human Caco-2 enterocyte sheet migration and proliferation. J. Cell. Physiol. 160: 491-501, 1994 PubMed: 94357990 Heinen, C.D., et al. Microsatellite instability in colorectal adenocareinoma cell lines that have full-length adenomatous polyposis coil protein. Cancer Res. 55: 4797-4799, 1995 PubMed: 96046575 GilberL, T., Rodriguez-Boulan, E. Induction of vacuolar apical compartments in the Caco-2 intestinal epithelial cell line. J. Cell Sci. 100: 451-458, 1991 PubMed: 92226167 Rigothier, M.C., et al. A new in vitro model of Entamoeba histolytica adhesion, using the human colon carcinoma cell line Caco-2: scanning electron microscopic study. Infect. Immun. 59: 4142-4146, 1991 PubMed: 92040089 Levin, M.S. Cellular retinol-binding proteins are determinants of retinol uptake and metabolism in stably transfected Caco-2 cells. J. Biol. Chem. 268: 8267-8276.1993 PubMed: 93216811 Trotter, P.J., and Storch, J. Fatty acid esterification during differentiation of the human intestinal cell line Caco-2. J. Biol. Chem. 268: 10017-10023, 1993 PubMed: 93252877 Santoro, I.M., Groden, J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997 PubMed:97164672 White, L.J., et al. Attachment and entry of recombinant norwalk virus eapsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996 PubMed: 96386543 Baier, L.J., et al. A polymorphism in the human intestinal fatty acid binding protein alters fatty acid transport across Caco-2 cells. J. Biol. Chem. 271: 10892-19896. 1996 PubMed: 96210027 Kutchera, W., et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93:4816-4820, 1996 PubMed: 96209815 Grindstaff, K.K. et al. Translational regulation of Na,K-ATPase alpha1 and beta1 polypeptide expression in epithelial cells. J. Biol. Chem. 271: 23211-23221, 1996 PubMed: 96394411 Hay, R. J., Caputo, J. L., and Macy, M. L. Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.

中文名称
人结直肠腺癌细胞
价格
Transmittal Fee
1300



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