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中国科学院细胞库细胞株信息

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细胞名称
Cell Name

A549人非小细胞肺癌细胞

描述
Description

该细胞系由D.J.Griad通过肺癌组织移植培养建系,患者为58岁白人男性。 A549能通过胞苷二磷酸胆碱途径合成含有高含量不饱和脂肪酸的卵磷脂。 在本库通过支原体检测。 在本库通过STR检测。

动物种别
Organism

性别
Gender

组织来源
Tissue and Cell Type

肺癌

形态
Morphology

上皮细胞

培养基和添加剂
Complete Growth Medium and Culture Conditions

F12K培养基(SIGMA,货号N3520,添加NaHCO3 2.5g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。

供应限制
Permits and Restrictions
A。仅供研究之用。

REFERENCE

Giard DJ , et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 Mayr GA , Freimuth P . A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365 Goodrum FD , Ornelles DA . The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383 St. Geme JW , et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Horikami SM , et al. The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222: 383-390, 1996. PubMed: 8806522 Huang S , et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475 Goodrum FD , et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Fang R , Aust AE . Induction of ferritin synthesis in human lung epithelial cells treated with crocidolite asbestos. Arch. Biochem. Biophys. 340: 369-375, 1997. PubMed: 9143343 Geiger T , et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 Evdokiou A , Cowled PA . Tumor-suppressive activity of the growth arrest-specific gene GAS1 in human tumor cell lines. Int. J. Cancer 75: 568-577, 1998. PubMed: 9466658 Giavedoni LD , Yilma T . Construction and characterization of replication-competent simian immunodeficiency virus vectors that express gamma interferon. J. Virol. 70: 2247-2251, 1996. PubMed: 8642649 Bartz SR , et al. Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G2 accumulation by a mechanism which differs from DNA damage checkpoint control. J. Virol. 70: 2324-2331, 1996. PubMed: 8642659 Garofalo R , et al. Transcriptional activation of the interleukin-8 gene by respiratory syncytial virus infection in alveolar epithelial cells: nuclear translocation of the RelA transcription factor as a mechanism producing airway mucosal inflammation. J. Virol. 70: 8773-8781, 1996. PubMed: 8971006 Jamaluddin M , et al. Inducible translational regulation of the NF-IL6 transcription factor by respiratory syncytial virus infection in pulmonary epithelial cells. J. Virol. 70: 1554-1563, 1996. PubMed: 8627674 Lewis JA , et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694 Lieber M , et al. A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int. J. Cancer 17: 62-70, 1976. PubMed: 175022 Acta Cell Biology Sinica 24(6):379-382,2002 Acta Biochimica et Biophysica Sinica 34(6):748-752, 2002 Chin J Cell Mol Immunol 18(4):332-334,2002 CHINA ONCOLOGY 12(5):395-398,2002 CHINA ONCOLOGY 12(5):441-443,2002 Chin J Oncol. 24(6):537-539,2002 Acta Biochimica et Biophysica Sinica 35(6):548-553,2003 Tumor 23(2):140-142,2003 Chin J Microbiol Immunol 23(4):288-291,2003 Chinese Journal of Cancer 22(2):171-174,2003 Suzhou University Journal of Medical Science 23(3):284-285,2003 The FASEB Journal 16:294,2002

中文名称
人非小细胞肺癌细胞
价格
Transmittal Fee
1100



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