首页  |  细胞库简介  |  细胞系目录  |  试剂类产品  |  细胞质量控制  |  订购和付款方式  |  管理团队  |  细胞培养基本方法  |  联系我们

中国科学院细胞库细胞株信息

点击订购此细胞株

细胞名称
Cell Name

U-937人组织细胞淋巴瘤细胞

描述
Description

U-937细胞系由Sundstrom和Nilsson在1974年建立,取材于患组织细胞淋巴瘤病人的胸水。 研究表明:U-937细胞能被人混合淋巴细胞培养上清,佛波脂、维生素D3、γ干扰素、肿瘤坏死因子和维甲酸诱导终末单核细胞分化。 该细胞免疫球蛋白产物和EB病毒表达为阴性。 该细胞表达Fas抗原且对TNF和抗-Fas抗体敏感。 在本库通过支原体检测。 在本库通过STR检测。

动物种别
Organism

性别
Gender

组织来源
Tissue and Cell Type

组织细胞淋巴瘤

形态
Morphology

单核细胞

培养基和添加剂
Complete Growth Medium and Culture Conditions

RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。

供应限制
Permits and Restrictions
A。仅供研究之用。

REFERENCE

Sundstrom C and Nilsson K. Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int. J. Cancer 17:565-577, 1976 PubMed: 76189427 Ralph P , et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890 Gene expression during normal and malignant differentiation. 143: London: Academic Press; 1985, pp. 57-72. International symposium on new trends in human immunology and cancer immunotherapy. Paris: Doin Editeurs; 1980, pp. 271-292. Koren HS , et al. In vitro activation of a human macrophage-like cell line. Nature 279: 328-331, 1979. PubMed: 450085 Gidlund M , et al. Natural killer cells kill tumour cells at a given stage of differentiation. Nature 292: 848-850, 1981. PubMed: 7266653 Olsson I , et al. Induction of differentiation of the human histiocytic lymphoma cell line U-937 by 1 alpha,25-dihydroxycholecalciferol. Cancer Res. 43: 5862-5867, 1983. PubMed:6315218 Morimoto H , et al. Overcoming tumor necrosis factor and drug resistance of human tumor cell lines by combination treatment with anti-Fas antibody and drugs or toxins. Cancer Res. 53: 2591-2596, 1993. PubMed: 7684321 Giovannangeli C , et al. Accessibility of nuclear DNA to triplex-forming oligonucleotides: The integrated HIV-1 provirus as a target. Proc. Natl. Acad. Sci. USA 94: 79-84, 1997. PubMed: 8990164 Brigino E , et al. Interleukin 10 is induced by recombinant HIV-1 Nef protein involving the calcium/calmodulin-dependent phosphodiesterase signal transduction pathway. Proc. Natl. Acad. Sci. USA 94: 3178-3182, 1997. PubMed: 9096366 Reid YA , et al. Cell Line Cross-contamination of U-937. J. Leukocyte Biol. 57: 804, 1995. PubMed: 7759961 Sundstrom C , Nilsson K . Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int. J. Cancer 17: 565-577, 1976. PubMed: 178611 Hay, R. J., Caputo, J. L, and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227. 1988. Fleming, D.O., Richardson, J. H., TuIis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control (1993), Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. (CDC) 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.

中文名称
人组织细胞淋巴瘤细胞
价格
Transmittal Fee
1400



您是第 14000877 位访问者,欢迎!
中国科学院典型培养物保藏委员会细胞库/中国科学院上海生命科学研究院细胞资源中心 版权所有
沪ICP备05033115号