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中国科学院细胞库细胞株信息

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细胞名称
Cell Name

A549人非小细胞肺癌细胞(2017年新引进)(复苏包邮;或可提供冻存管)

描述
Description

人非小细胞肺癌细胞A549来源于58岁的白人男性肺癌组织,由D.J. Giard等人建立于1972年。M. Lieber发现A549可以通过胞苷二磷酸胆碱途径合成高比例的不饱和脂肪酸卵磷脂。该细胞复苏后不耐低温和长时间运输,复苏后沿途最低温度需高于10摄氏度且运输路途不可过长。冬天常温邮寄A549细胞时会有细胞脱落、坏死等现象,请尽量选用干冰冷链快递。通过本库支原体检测。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测无误。STR鉴定结果为:Amelogenin: X,Y;CSF1PO: 10,12;D13S317: 11;D16S539: 11,12;D5S818: 11;D7S820: 8,11;THO1: 8,9.3;TPOX: 8,11;vWA: 14

动物种别
Organism

性别
Gender

男性,58岁

组织来源
Tissue and Cell Type

肺部

形态
Morphology

上皮样,贴壁生长

培养基和添加剂
Complete Growth Medium and Culture Conditions

A549细胞完全培养液配方:
F-12K(Invitrogen,21127-022) 89 ml
FBS(Gibco) 10 ml
Glutamax (Invitrogen, 35050061) 1 ml

参考传代周期:每周2-3次
参考传代比例:1 : 3
备注:培养时保持细胞密度在6000 到 60000 cells/平方厘米,不要超过70000 cells/平方厘米
参考换液频率:每周2-3次
冻存液配方:完全培养液95%,DMSO 5%
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。

供应限制
Permits and Restrictions
A。仅供研究之用。

REFERENCE

Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365 Goodrum FD, Ornelles DA. The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed: 8985383 St. Geme JW, et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 8892830 Horikami SM, et al. The Sendai virus V protein interacts with the NP protein to regulate viral genome RNA replication. Virology 222: 383-390, 1996. PubMed: 8806522 Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475 Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Fang R, Aust AE. Induction of ferritin synthesis in human lung epithelial cells treated with crocidolite asbestos. Arch. Biochem. Biophys. 340: 369-375, 1997. PubMed: 9143343 Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 Evdokiou A, Cowled PA. Tumor-suppressive activity of the growth arrest-specific gene GAS1 in human tumor cell lines. Int. J. Cancer 75: 568-577, 1998. PubMed: 9466658 Giavedoni LD, Yilma T. Construction and characterization of replication-competent simian immunodeficiency virus vectors that express gamma interferon. J. Virol. 70: 2247-2251, 1996. PubMed: 8642649 Bartz SR, et al. Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G2 accumulation by a mechanism which differs from DNA damage checkpoint control. J. Virol. 70: 2324-2331, 1996. PubMed: 8642659 Garofalo R, et al. Transcriptional activation of the interleukin-8 gene by respiratory syncytial virus infection in alveolar epithelial cells: nuclear translocation of the RelA transcription factor as a mechanism producing airway mucosal inflammation. J. Virol. 70: 8773-8781, 1996. PubMed: 8971006 Jamaluddin M, et al. Inducible translational regulation of the NF-IL6 transcription factor by respiratory syncytial virus infection in pulmonary epithelial cells. J. Virol. 70: 1554-1563, 1996. PubMed: 8627674 Lewis JA, et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694 Lieber M, et al. A continuous tumor-cell line from a human lung carcinoma with properties of type II alveolar epithelial cells. Int. J. Cancer 17: 62-70, 1976. PubMed: 175022

细胞说明书

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中文名称
人非小细胞肺癌细胞(2017年新引进)(复苏包邮;或可提供冻存管)
价格
Transmittal Fee
1100


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