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中国科学院细胞库细胞株信息

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细胞名称
Cell Name

Jurkat, Clone E6-1人白血病T淋巴细胞(2018年新引进)(复苏包邮;或可提供冻存管)

描述
Description

人白血病T淋巴细胞,Jurkat细胞由Schneider建立,来源于一个14岁的男孩的外周血。该克隆是 Jurkat-FHCRC细胞株的一个克隆(Jurkat的一个衍生株),经佛波酯(phorbol esters)和外源凝集素(lectins)或抗T3单克隆抗体(需要两种物质共同诱导)诱导后可产生大量IL-2。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测结果无误。STR结果如下:D5S818: 9;D13S317: 8,12;D7S820: 8,12;D16S539: 11;vWA: 18;THO1: 6,9.3;Amelogenin: X,Y;TPOX: 8,10;CSF1PO: 11,12

动物种别
Organism

性别
Gender

男性

组织来源
Tissue and Cell Type

外周血

形态
Morphology

成淋巴细胞状,悬浮生长

培养基和添加剂
Complete Growth Medium and Culture Conditions

1. 人白血病T淋巴细胞Jurkat, Clone E6-1完全培养液配方(100 ml)
RPMI 1640 Medium (Invitrogen, 11875-093) 88 ml
FBS (Gibco) 10 ml
Glutamax(invitrogen 35050-061) 1 ml
Sodium Pyruvate 100 mM Solution (invitrogen 11360070) 1 ml
参考传代周期:不超过300万细胞 /mL,建议将活细胞密度控制在10万-100万/mL之间
参考传代密度:10万活细胞/mL
参考换液频率:2-3天
冻存液:完全培养液95%,DMSO 5%
细胞状态:悬浮生长
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。

2. 注意事项:
a) 该细胞为悬浮细胞,根据培养经验以及客户的反馈,传代时使用【半换液法】对细胞状态较为有利,因此我库建议您使用【半换液法】进行传代。同时,您在收到细胞后,请不要通过离心的方式收集细胞,可以直接向培养瓶中添加等体积的新鲜培养液,然后将细胞吹打均匀后移入两个新的T25培养瓶中继续培养即可。
b) 细胞对血清质量较为敏感,我库建议您使用进口大品牌优质血清进行培养。
c) 该细胞对细胞密度较为敏感,培养、传代时请注意保持细胞密度在合适的范围。

供应限制
Permits and Restrictions
A。仅供研究之用。

REFERENCE

Weiss A, et al. The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL-2 production reflects events occurring at a pre-translational level. J. Immunol. 133: 123-128, 1984. PubMed: 6327821 Gillis S, Watson J. Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line. J. Exp. Med. 152: 1709-1719, 1980. PubMed: 6778951 Berninghausen O, Leippe M. Necrosis versus apoptosis as the mechanism of target cell death induced by Entamoeba histolytica. Infect. Immun. 65: 3615-3621, 1997. PubMed:9284127 Churchill MJ, et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194 Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663 Tiffany HL, et al. Enhanced expression of the eosinophil-derived neurotoin ribonuclease (RNS2) gene requires interaction between the promoter and intron. J. Biol. Chem. 271: 12387-12393, 1996. PubMed: 8647842 Chan YJ, et al. Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus. J. Virol. 70: 8590-8605, 1996. PubMed: 8970984 Kung SH, Medveczky PG. Identification of a herpesvirus saimiri cis-acting DNA fragment that permits stable replication of episomes in transformed T cells. J. Virol. 70: 1738-1744, 1996. PubMed: 8627695 Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082 Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306 Linette GP, et al. Cross talk between cell death and cell cycle progression: BCL-2 regulates NFAT-mediated activation. Proc. Natl. Acad. Sci. USA 93: 9545-9552, 1996. PubMed: 8790367 Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538 Yang RY, et al. Expression of galectin-3 modulates T-cell growth and apoptosis. Proc. Natl. Acad. Sci. USA 93: 6737-6742, 1996. PubMed: 8692888 Gibson S, et al. Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK. J. Biol. Chem. 271: 7079-7083, 1996. PubMed: 8636141 Ponton A, et al. The CD95 (APO-1/Fas) receptor activates NF-kappaB independently of its cytotoxic function. J. Biol. Chem. 271: 8991-8995, 1996. PubMed: 8621545 August A, Dupont B. Association between mitogen-activated protein kinase and the zeta chain of the T cell receptor (TcR) with the SH2,3 domain of p56lck. J. Biol. Chem. 271: 10054-10059, 1996. PubMed: 8626561 Kotanides H, Reich NC. Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene. J. Biol. Chem. 271: 25555-25561, 1996. PubMed:8810328 Hartley D, Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J. Biol. Chem. 271: 21939-21943, 1996. PubMed: 8702998 Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022 Schneider U, et al. Characterization of EBV-genome negative "null" and "T" cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma. Int. J. Cancer 19: 621-626, 1977. PubMed: 68013 Ronald Wange, personal communication

细胞说明书

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中文名称
人白血病T淋巴细胞(2018年新引进)(复苏包邮;或可提供冻存管)
价格
Transmittal Fee
1100


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