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中国科学院细胞库细胞株信息

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细胞名称
Cell Name

PC-3人前列腺癌细胞(2013年新引进)(复苏包邮;或可提供冻存管)

描述
Description

人前列腺癌细胞PC-3来源于一名62岁IV级前列腺癌白人男性患者的骨髓转移灶。此细胞酸性磷酸酶(acid phosphatase)和5-α-睾丸激素还原酶(testosterone-5-alpha reductase) 活性表达低。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测无误。STR鉴定结果为:Amelogenin: X;CSF1PO: 11;D13S317: 11;D16S539: 11;D5S818: 13;D7S820: 8,11;THO1: 6,7;TPOX: 8,9;vWA: 17

动物种别
Organism

人;白人

性别
Gender

男性,62岁

组织来源
Tissue and Cell Type

前列腺;分离自转移灶:骨

形态
Morphology

上皮样,贴壁生长;该细胞在软琼脂中成蔟生长,并可适应悬浮培养)

培养基和添加剂
Complete Growth Medium and Culture Conditions

人前列腺癌细胞PC-3细胞完全培养液配方(100 ml):
   Ham's F-12K (Kaighn's) Medium (Invitrogen, 21127-022) 89 ml
   FBS (Gibco) 10 ml
Glutamax (Invitrogen, 35050061) 1 ml
参考传代周期:3-4天
参考传代比例:1:3—1:6
参考换液频率:每周2-3次
冻存液配方:完全培养液95%,DMSO 5%
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。

供应限制
Permits and Restrictions
A。仅供研究之用。

REFERENCE

Kaighn ME, et al. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest. Urol. 17: 16-23, 1979. PubMed: 447482 Chen TR. Chromosome identity of human prostate cancer cell lines, PC-3 and PPC-1. Cytogenet. Cell Genet. 62: 183-184, 1993. PubMed: 8428522 Ohnuki Y, et al. Chromosomal analysis of human prostatic adenocarcinoma cell lines. Cancer Res. 40: 524-534, 1980. PubMed: 7471073 Sheng S, et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 8876194 Umekita Y, et al. Human prostate tumor growth in athymic mice: inhibition by androgens and stimulation by finasteride. Proc. Natl. Acad. Sci. USA 93: 11802-11807, 1996. PubMed: 8876218 Carter RE, et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 8570628 Nupponen NN, et al. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. PubMed: 9460501 Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 Su ZZ, et al. Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family. Proc. Natl. Acad. Sci. USA 93: 7252-7257, 1996. PubMed: 8692978

细胞说明书

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中文名称
人前列腺癌细胞(2013年新引进)(复苏包邮;或可提供冻存管)
价格
Transmittal Fee
1100


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