首页  |  细胞库简介  |  细胞系目录  |  试剂类产品  |  细胞质量控制  |  订购和付款方式  |  管理团队  |  细胞培养基本方法  |  联系我们

中国科学院细胞库细胞株信息

点击订购此细胞株

细胞名称
Cell Name

HuH-7人肝癌细胞(复苏包邮;或可提供冻存管)

描述
Description

有报道称人肝癌细胞HuH-7可以无血清培养,会在培养基内分泌许多血清蛋白和alpha phyto蛋白。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测结果无误。STR结果如下:D5S818: 12;D13S317: 10,11;D7S820: 11;D16S539: 10;vWA: 16,18;THO1: 7;Amelogenin: X;TPOX: 8,11;CSF1PO: 11

动物种别
Organism

性别
Gender

组织来源
Tissue and Cell Type

肝脏

形态
Morphology

上皮样,贴壁生长

培养基和添加剂
Complete Growth Medium and Culture Conditions

DMEM (Invitrogen, 11960-044) 88ml
FBS (Gibco) 10ml
Glutamax (Invitrogen, 35050-061) 1ml
Non-essential Amino Acids, 100X (Invitrogen, 11140-050) 1ml
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。

供应限制
Permits and Restrictions
A。仅供研究之用。

REFERENCE

Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs.Randall G,Grakoui A,Rice CM Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):235-40 Highly permissive cell lines for subgenomic and genomic hepatitis C virus RNA replication.Blight KJ,McKeating JA,Rice CM J Virol. 2002 Dec;76(24):13001-14 Replication of subgenomic hepatitis C virus RNAs in a hepatoma cell line.Lohmann V,K�rner F,Koch J,Herian U,Theilmann L,Bartenschlager R Science. 1999 Jul 2;285(5424):110-3 Establishment and characteristics of human hepatocellular carcinoma cells with metastasis to lymph nodes.Seki S,Kitada T,Kawada N,Sakaguchi H,Kadoya H,Nakatani K,Satake K,Kuroki T Hepatogastroenterology. 1999 Sep-Oct;46(29):2812-7 Hormonal control of alpha-fetoprotein secretion in human hepatoma cell lines proliferating in chemically defined medium.Nakabayashi H,Taketa K,Yamane T,Oda M,Sato J Cancer Res. 1985 Dec;45(12 Pt 1):6379-83 Phenotypical stability of a human hepatoma cell line, HuH-7, in long-term culture with chemically defined medium.Nakabayashi H,Taketa K,Yamane T,Miyazaki M,Miyano K,Sato J Gann. 1984 Feb;75(2):151-8 Growth of human hepatoma cells lines with differentiated functions in chemically defined medium.Nakabayashi H,Taketa K,Miyano K,Yamane T,Sato J Cancer Res. 1982 Sep;42(9):3858-63

细胞说明书

点击查看

中文名称
人肝癌细胞(复苏包邮;或可提供冻存管)
价格
Transmittal Fee
1100


您是第 13907431 位访问者,欢迎!
中国科学院典型培养物保藏委员会细胞库/中国科学院上海生命科学研究院细胞资源中心 版权所有
沪ICP备05033115号