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中国科学院细胞库细胞株信息

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细胞名称
Cell Name

MS751人子宫颈表皮癌细胞(2014年新引进)(复苏包邮;或可提供冻存管)

描述
Description

人子宫颈表皮癌细胞MS751由J. Sykes于1974年建立,来源于一位47岁白人女性。该细胞含有人乳头状瘤病毒18(HPV-18)序列。最近发现MS751细胞包含部分来源于E6/E7区域的HPV-45基因序列,表达poly(A)+RNA的形式。生物安全等级:二级。每个批次均通过本库支原体检测,结果为阴性。经本库STR检测结果无误。STR结果如下:D5S818: 12;D13S317: 12;D7S820: 9,11;D16S539: 11;vWA: 16;THO1: 6;Amelogenin: X;TPOX: 8;CSF1PO: 11,12

动物种别
Organism

人;白人

性别
Gender

女性,47岁

组织来源
Tissue and Cell Type

子宫颈;分离自转移灶:淋巴结

形态
Morphology

上皮样,贴壁生长

培养基和添加剂
Complete Growth Medium and Culture Conditions

Eagle MEM (Invitrogen, 11090081) 88 ml
FBS (Gibco) 10 ml
Glutamax(invitrogen 35050061) 1 ml
Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml
气相:空气,95%;二氧化碳,5%。温度:37摄氏度。

供应限制
Permits and Restrictions
A。仅供研究之用。

REFERENCE

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Pater MM, Pater A. Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix. Virology 145: 313-318, 1985. PubMed: 2992153 Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 Geisbill J, et al. Detection and characterization of human papillomavirus type 45 DNA in the cervical carcinoma cell line MS751. J. Gen. Virol. 78: 655-658, 1997. PubMed: 9049418 Smith KJ, et al. The APC gene product in normal and tumor cells. Proc. Natl. Acad. Sci. USA 90: 2846-2850, 1993. PubMed: 8385345 Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/OD/ohs/biosfty/bmbl5/bmbl5toc.htm

细胞说明书

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中文名称
人子宫颈表皮癌细胞(2014年新引进)(复苏包邮;或可提供冻存管)
价格
Transmittal Fee
1100


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