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中国科学院细胞库细胞株信息

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细胞名称
Cell Name

293 [HEK-293]停止供应人胚肾细胞

描述
Description

尽管较早的报道说这株细胞包含腺病毒5 DNA病毒基因组的左端和右端,现在已经清楚只存在左端序列。这株细胞适于滴定人腺病毒。细胞表达一种不寻常的由整联蛋白beta-1亚基和玻联蛋白alpha-v亚基组成的玻联蛋白细胞表面受体。Ad5插入片段进行了克隆和测序,结果表明碱基1-4344插入到了19号染色体(19q13.2)。 在本库通过支原体检测。 在本库通过STR检测。

动物种别
Organism

性别
Gender

***

组织来源
Tissue and Cell Type

器官:胚胎肾细胞类型:腺病毒5 DNA 转染

形态
Morphology

上皮细胞

培养基和添加剂
Complete Growth Medium and Culture Conditions

DMEM培养基(GIBCO,货号12800017,添加NaHCO3 1.5g/L),90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。

供应限制
Permits and Restrictions
A。仅供研究之用。

REFERENCE

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PubMed: 8702465 32914: Oppermann M, et al. Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. Proc. Natl. Acad. Sci. USA 93: 7649-7654, 1996. PubMed: 8755530 32921: Xia Y, et al. Nitric oxide synthase generates superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury. Proc. Natl. Acad. Sci. USA 93: 6770-6774, 1996. PubMed: 8692893 32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224 32971: Uebele VN, et al. Functional differences in Kv1.5 currents expressed in mammalian cell lines are due to the presence of endogenous Kvbeta2.1 subunits. J. Biol. Chem. 271: 2406-2412, 1996. PubMed: 8576199 33003: Abell A, et al. 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中文名称
人胚肾细胞
价格
Transmittal Fee
1100



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