| 细胞名称 Cell Name |
Jurkat, Clone E6-1 |
| 目录号 Serial |
TCHU123 |
| 标识符 Identifier |
CSTR:19375.09.3101HUMTCHU123 |
| 描述 Description |
Jurkat, Clone E6-1细胞是Jurkat-FHCRC细胞株(Jurkat细胞株的衍生)的一个克隆株，细胞系是从一名14岁男性急性t细胞白血病患者的外周血中建立的。该细胞系可用于免疫系统疾病的研究以及免疫学和免疫肿瘤学的研究。。Jurkat细胞株经佛波酯和外源凝集素或抗T3单克隆抗体(两种类型的诱导都需要)诱导后可产生大量IL-2。 细胞在本库通过支原体检测阴性（可供检测报告）。 细菌检测结果：阴性；真菌检测结果：阴性 细胞在本库通过STR检测无误（可供检测报告）。 STR鉴定结果： Amelogenin: X,Y D5S818:9 D13S317: 8,12 D7S820: 8,12 D16S539: 11 vWA: 18 TH01: 6,7,9.3 TPOX: 8,10 CSF1PO: 11,12 D19S433: 14,15.2 D21S11: 31.2,33.2 D18S51: 13,21 |
| 动物种别 Organism |
人 |
| 性别 Gender |
男性 |
| 组织来源 Tissue and Cell Type |
T淋巴细胞；急性T细胞白血病 |
| 形态 Morphology |
淋巴母细胞, 悬浮生长 |
| 培养基和添加剂 Complete Growth Medium and Culture Conditions |
人白血病T淋巴细胞Jurkat, Clone E6-1完全培养液（目录号：MD04）组分： RPMI 1640培养基(GIBCO货号：11875093)，90%；优质胎牛血清（GIBCO），10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 培养参考：细胞传代比例1:2，传代周期2-3天 冻存液配方：完全培养液92%，DMSO 8% 细胞运输方式：活细胞可保温运输或干冰冻存管。 注意事项： （1）细胞除首次传代需离心收集细胞，后续细胞换液或传代时建议使用半换液法，即换液或传代时待细胞自然沉降后，弃去一半旧培养 液，之后可直接向培养瓶中添加等体积的新鲜培养液，然后将细胞吹打均匀或移入两个新的T25 培养瓶中（1:2传代），补充新的完 全 培养基至8-10ml/瓶即可。 （2）细胞悬浮聚团生长，注意细胞密度，及时换液、传代。 （3）细胞对胎牛血清质量比较敏感，建议使用高质量胎牛血清。 |
| 供应限制 Permits and Restrictions |
A。仅供研究之用。 |
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| 细胞说明书 | 登录后下载 |
| 中文名称 | 人T淋巴细胞白血病细胞（顺丰包邮, 或可供冻存管） |
|
价格 |
1200 |
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联系人：吴军
E-mail：ecell@sibs.ac.cn
地址：中国科学院细胞库，徐汇区岳阳路320号，上海200031
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传真：021-54921358
联系人：顾老师
E-mail：scell@sibcb.ac.cn
地址：中国科学院细胞库，徐汇区岳阳路320号，上海200031
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