细胞名称 Cell Name |
293FT [HEK 293FT] |
目录号 Serial |
SCSP-5212 |
标识符 Identifier |
CSTR:19375.09.3101HUMSCSP5212 |
描述 Description |
该细胞来源于用SV40大T抗原转化的人胚胎肾细胞293F(F是Fast-growth的首字母),是一种快速生长、高度可转染的克隆分离株。它是制备高滴度慢病毒的良好宿主,能制造高滴度的慢病毒,能够稳定表达pCMVSPORT6TAg.neo 质粒中的SV40大T抗原,且大T抗原的表达受到人类巨细胞病毒(CMV)启动子的控制,表达水平较高。此外,该细胞还可稳定表达新霉素抗性基因,且新霉素抗性基因的表达受到SV40增强子/启动子的控制,因此可用含有0.5 mg/ml G418的培养基中维持培养。 细胞质控检测(可提供检测报告): 1) 支原体检测结果:阴性 2) 细菌检测结果:阴性 3) 真菌检测结果:阴性 4) 细胞SV40大T抗原检测:SV40大T抗原阳性 5) STR鉴定结果: Amelogenin: X, X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11, 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11, 11 CSF1PO: 7, 12 D19S433: 15, 18D21S11: 30.2, 30.2 D18S51: 17, 18 D6S1043: 11, 11 D3S1358: 15, 17 Penta D: 9, 9 D2S441: 11, 15 D8S1179: 12, 14 Penta E: 7, 15 D12S391: 19, 21 D2S1338: 19, 19 FGA: 23, 23 |
动物种别 Organism |
人,白人 |
性别 Gender |
女性 |
组织来源 Tissue and Cell Type |
胎儿,胚肾 |
形态 Morphology |
上皮样,贴壁生长 |
培养基和添加剂 Complete Growth Medium and Culture Conditions |
人胚肾细胞293FT [HEK293-FT]完全培养液(SCSP-688)配方: DMEM培养基(Gibco, 货号11995065): 88% 胎牛血清FBS(Gibco): 10% 非必需氨基酸溶液NEAA (Gibco,货号11140050): 1% GlutaMAX (Gibco,货号35050061): 1% 添加G418(Gibco,货号10131027)至终浓度0.5 mg/ml 参考传代比例:1:3 参考传代周期:2-4天 参考换液频率:每2-3天换1次液 冻存液配方:完全培养液90%,DMSO 10% 培养条件:气相:空气95%,二氧化碳5%;温度:37摄氏度。 细胞运输方式:活细胞保温运输或冻存管干冰运输。 注意事项: 1. DMEM培养基中已含有4 mM L-glutamine, 但由于L-glutamine会随着时间的推移而缓慢降解,所以我们额外添加 2 mM GlutaMAX。 2. 细胞复苏时可选用不含G418的完全培养基,第二天更换为含有0.5 mg/ml G418 的新鲜完全培养基。 |
供应限制 Permits and Restrictions |
A。仅供研究之用。 |
REFERENCE | Chen, C., and Okayama, H. High-Efficiency Transformation of Mammalian Cells by Plasmid DNA.Mol.Cell.Biol.1987, 7: 2745-2752 Chu, G., Hayakawa, H., and Berg, P. Electroporation for the Efficient Transfection of Mammalian Cells with DNA.Nucleic Acids Res.1987, 15: 1311-1326 Felgner, P. L., Holm, M., and Chan, H. Cationic Liposome Mediated Transfection.Proc.West.Pharmacol.Soc.1989, 32: 115-121 Felgner, P. L. a., and Ringold, G. M. Cationic Liposome-Mediated Transfection.Nature. 1989, 337: 387- 388 Graham, F. L., Smiley, J., Russell, W. C, et al. Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5.J. Gen.Virol. 1977, 36: 59-74 Harrison, T., Graham, F., and Williams, J. Host-range Mutants of Adenovirus Type 5 Defective for Growth in HeLa Cells.Virology. 1977, 77: 319-329 Naldini, L., Blomer, U., Gage, F. H., et al. Efficient Transfer, Integration, and Sustained Long-Term Expression of the Transgene in Adult Rat Brains Injected with a Lentiviral Vector.Proc.Natl.Acad.Sci.USA. 1996, 93: 11382-11388 Shigekawa, K., and Dower, W. J. Electroporation of Eukaryotes and Prokaryotes: A General Approach to the Introduction of Macromolecules into Cells.BioTechniques. 1988, 6: 742-751 Southern, P. J., and Berg, P. Transformation of Mammalian Cells to Antibiotic Resistance with a Bacterial Gene Under Control of the SV40 Early Region Promoter.J. Molec.Appl.Gen.1982, 1: 327-339 Wigler, M., Silverstein, S., Lee, L.-S., et al. Transfer of Purified Herpes Virus Thymidine Kinase Gene to Cultured Mouse Cells.Cell. 1977, 11: 223-232 |
细胞说明书 | 登录后下载 |
中文名称 | 人胚肾细胞(活细胞顺丰包邮;或可提供冻存管) |
价格 |
1600 |
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